The Multiplex qPCR Detection kits contain an optimized Primer-Probe set with primers and probes for human RNaseP and Nl and N2 regions of the nucleocapsid gene of SARS-CoV-2 Virus.
High-Accuracy (%99) Worldwide Acceptance
Enzyme Include Options
Minimize Human Error & Contamination
High Specificity & Sensitivity
Optimized primer/probe sequences to minimize false - positives
- to check for RNA quality to minimize false-negatives
- to verify the enzyme and other reagents
l00% inclusivity of published SARS-CoV-2 sequences in unmutated targeted regions
Good Reasons to Choose our qPCR Test Kits & PCR Analysis Devices
SARS-CoV-2 (COVID-19) tests for the presence and absence of ribonucleic acid (RNA) of strain SARS-CoV-2 coronavirus. Test includes a Primer/Probe set that contains primers and probes for viral N1 and N2 regions. The primers and probes for viral N1 and N2 regions specifically identify two distinct regions on the nucleocapsid (N) gene of the SARS-CoV-2 virus. These primers and probes do not recognize the SARS-CoV virus, whose genetic structure is highly similar to SARS-CoV-2.
The internal control (primer/probe set for human RNaseP gene) is used to identify possible qPCR inhibition, confirm the integrity of the reagents, and verify the quality of nucleic acid extraction from patient material. RNaseP primers and probes included in the kit recognize the human RNaseP gene.
Test includes a positive control, which is labelled as “Positive Control”. The Positive Control is a synthetic DNA which contains the target regions of the N1, N2 and RNaseP genes that are amplified with the primer-probe sets included in the test kit.
The test has been developed to be used as a single one-step reverse transcription qPCR test for each patient sample, utilizing PCR amplification of the targets (N1, N2 and RNaseP) by FAM, HEX and Cy5 labelled probes. The reaction time is approximately 1 hour. The test contains internal controls that monitor the performance of the test.